Jet interactions with a giant molecular cloud in the Galactic centre and ejection of hypervelocity stars

The hypervelocity OB stars in the Milky Way Galaxy were ejected from the central regions some 10-100 million years ago. We argue that these stars, {as well as many more abundant bound OB stars in the innermost few parsecs,} were generated by the interactions of an AGN jet from the central black hole with a dense molecular cloud. Considerations of the associated energy and momentum injection have broader implications for the possible origin of the Fermi bubbles and for the enrichment of the intergalactic medium.Comment: 4 pages, 1 figure. Astronomy and Astrophysics Letters, in pres

Magnetic fileds of coalescing neutron stars and the luminosity function of short gamma-ray burst

Coalescing neutron star binaries are believed to be the most reliable sources for ground-based detectors of gravitational waves and likely progenitors of short gamma-ray bursts. In the process of coalescence, magnetic fields of neutron stars can induce interesting observational manifestations and affect the form of gravitational wave signal. In this papaer we use the population synthesis method to model the expected distribution of neutron star magnetic fields during the coalescence under different assumptions on the initial parameters of neutron stars and their magnetic field evolution. We discuss possible elecotrmagnetic phenomena preceding the coalescence of magnetized neutron star binaries and the effect of magnetic field on the gravitational wave signal. We find that a log-normal (Gaussian in logarithms) distribution of the initial magnetic fields of neutron stars, which agrees with observed properties of radio pulsars, produces the distribution of the magnetic field energy during the coalescence that adequately describes the observed luminosity function of short gamma-ray bursts under different assumptions on the field evolution and initial parameters of neutron stars. This agreement lends further support to the model of coalescing neutron star binaries as progenitors of gamma-ray bursts.Comment: v.2, LATEX, 25 pages, inc. 7 ps figures, Astron. Lett., in press. Typos corrected, reference adde

An Eccentric Binary Millisecond Pulsar in the Galactic Plane

Binary pulsar systems are superb probes of stellar and binary evolution and the physics of extreme environments. In a survey with the Arecibo telescope, we have found PSR J1903+0327, a radio pulsar with a rotational period of 2.15 ms in a highly eccentric (e = 0.44) 95-day orbit around a solar mass companion. Infrared observations identify a possible main-sequence companion star. Conventional binary stellar evolution models predict neither large orbital eccentricities nor main-sequence companions around millisecond pulsars. Alternative formation scenarios involve recycling a neutron star in a globular cluster then ejecting it into the Galactic disk or membership in a hierarchical triple system. A relativistic analysis of timing observations of the pulsar finds its mass to be 1.74+/-0.04 Msun, an unusually high value.Comment: 28 pages, 4 figures inc Supplementary On-Line Material. Accepted for publication in Science, published on Science Express: 10.1126/science.115758

New Limits on Radio Emission from X-ray Dim Isolated Neutron Stars

We have carried out a search for radio emission at 820 MHz from six X-ray dim isolated neutron stars with the Robert C. Byrd Green Bank Radio Telescope. No transient or pulsed emission was found using fast folding, fast Fourier transform, and single-pulse searches. The corresponding flux limits are about 0.01 mJy for pulsed emission, depending on the integration time for the particular source and assuming a duty cycle of 2%, and 20 mJy for single dispersed pulses. These are the most sensitive limits to date on radio emission from X-ray dim isolated neutron stars. There is no evidence for isolated radio pulses, as seen in a class of neutron stars known as rotating radio transients. Our results imply that either the radio luminosities of these objects are lower than those of any known radio pulsars, or they could simply be long-period nearby radio pulsars with high magnetic fields beaming away from the Earth. To test the latter possibility, we would need around 40 similar sources to provide a 1 sigma probability of at least one of them beaming toward us. We also give a detailed description of our implementation of the Fast Folding Algorithm.Comment: 16 pages, 8 figures, 3 tables, accepted to Ap

Arecibo Pulsar Survey Using ALFA. I. Survey Strategy and First Discoveries

We report results from the initial stage of a long-term pulsar survey of the Galactic plane using the Arecibo L-band Feed Array (ALFA), a seven-beam receiver operating at 1.4 GHz with 0.3 GHz bandwidth. The search targets Galactic latitudes |b| < 5 deg in the longitude ranges 32 deg < l < 77 deg and 168 deg < l < 77 deg. Data discussed here were collected over a 100 MHz passband centered on 1.42 GHz using a spectrometer that recorded 256 channels every 64 microsec. In a preliminary, standard period-DM analysis, we have detected 29 previously known pulsars and discovered 11 new ones. One of these, with a period of 69 ms and a low characteristic age of 82 kyr, is a plausible candidate for association with the unidentified EGRET source 3EG J1928+1733. Another is a non-recycled pulsar in a relativistic binary with orbital period of 3.98 hr. We also search the data for isolated dispersed pulses, a technique that yielded discovery of an extremely sporadic radio emitter with a spin period of 1.2 s. Simulations we have carried out indicate that about 1000 new pulsars will be found in the ALFA survey. In addition to providing a large sample for use in population analyses and for probing the magnetoionic interstellar medium, the survey maximizes the chances of finding rapidly spinning millisecond pulsars and pulsars in compact binary systems. Our search algorithms will exploit the multiple data streams from ALFA to discriminate between radio frequency interference and celestial signals, including pulsars and possibly new classes of transient radio sources.Comment: 10 pp, 9 figures, accepted by the Astrophysical Journa

Role of Esrrg in the Fibrate-Mediated Regulation of Lipid Metabolism Genes in Human ApoA-I Transgenic Mice

We have used a new ApoA-I transgenic mouse model to identify by global gene expression profiling, candidate genes that affect lipid and lipoprotein metabolism in response to fenofibrate treatment. Multilevel bioinformatical analysis and stringent selection criteria (2-fold change, 0% false discovery rate) identified 267 significantly changed genes involved in several molecular pathways. The fenofibrate-treated group did not have significantly altered levels of hepatic human APOA-I mRNA and plasma ApoA-I compared with the control group. However, the treatment increased cholesterol levels to 1.95-fold mainly due to the increase in high-density lipoprotein (HDL) cholesterol. The observed changes in HDL are associated with the upregulation of genes involved in phospholipid biosynthesis and lipid hydrolysis, as well as phospholipid transfer protein. Significant upregulation was observed in genes involved in fatty acid transport and β-oxidation, but not in those of fatty acid and cholesterol biosynthesis, Krebs cycle and gluconeogenesis. Fenofibrate changed significantly the expression of seven transcription factors. The estrogen receptor-related gamma gene was upregulated 2.36-fold and had a significant positive correlation with genes of lipid and lipoprotein metabolism and mitochondrial functions, indicating an important role of this orphan receptor in mediating the fenofibrate-induced activation of a specific subset of its target genes.National Institutes of Health (HL48739 and HL68216); European Union (LSHM-CT-2006-0376331, LSHG-CT-2006-037277); the Biomedical Research Foundation of the Academy of Athens; the Hellenic Cardiological Society; the John F Kostopoulos Foundatio

Transitions between Inherent Structures in Water

The energy landscape approach has been useful to help understand the dynamic properties of supercooled liquids and the connection between these properties and thermodynamics. The analysis in numerical models of the inherent structure (IS) trajectories -- the set of local minima visited by the liquid -- offers the possibility of filtering out the vibrational component of the motion of the system on the potential energy surface and thereby resolving the slow structural component more efficiently. Here we report an analysis of an IS trajectory for a widely-studied water model, focusing on the changes in hydrogen bond connectivity that give rise to many IS separated by relatively small energy barriers. We find that while the system \emph{travels} through these IS, the structure of the bond network continuously modifies, exchanging linear bonds for bifurcated bonds and usually reversing the exchange to return to nearly the same initial configuration. For the 216 molecule system we investigate, the time scale of these transitions is as small as the simulation time scale ($\approx 1$ fs). Hence for water, the transitions between each of these IS is relatively small and eventual relaxation of the system occurs only by many of these transitions. We find that during IS changes, the molecules with the greatest displacements move in small ``clusters'' of 1-10 molecules with displacements of $\approx 0.02-0.2$ nm, not unlike simpler liquids. However, for water these clusters appear to be somewhat more branched than the linear ``string-like'' clusters formed in a supercooled Lennar d-Jones system found by Glotzer and her collaborators.Comment: accepted in PR

High throughput mutagenesis for identification of residues regulating human prostacyclin (hIP) receptor

The human prostacyclin receptor (hIP receptor) is a seven-transmembrane G protein-coupled receptor (GPCR) that plays a critical role in vascular smooth muscle relaxation and platelet aggregation. hIP receptor dysfunction has been implicated in numerous cardiovascular abnormalities, including myocardial infarction, hypertension, thrombosis and atherosclerosis. Genomic sequencing has discovered several genetic variations in the PTGIR gene coding for hIP receptor, however, its structure-function relationship has not been sufficiently explored. Here we set out to investigate the applicability of high throughput random mutagenesis to study the structure-function relationship of hIP receptor. While chemical mutagenesis was not suitable to generate a mutagenesis library with sufficient coverage, our data demonstrate error-prone PCR (epPCR) mediated mutagenesis as a valuable method for the unbiased screening of residues regulating hIP receptor function and expression. Here we describe the generation and functional characterization of an epPCR derived mutagenesis library compromising >4000 mutants of the hIP receptor. We introduce next generation sequencing as a useful tool to validate the quality of mutagenesis libraries by providing information about the coverage, mutation rate and mutational bias. We identified 18 mutants of the hIP receptor that were expressed at the cell surface, but demonstrated impaired receptor function. A total of 38 non-synonymous mutations were identified within the coding region of the hIP receptor, mapping to 36 distinct residues, including several mutations previously reported to affect the signaling of the hIP receptor. Thus, our data demonstrates epPCR mediated random mutagenesis as a valuable and practical method to study the structurefunction relationship of GPCRs. © 2014 Bill et al